Ap biology enzyme lab pdf

Enzyme reaction rates using toothpickase introduction. As could be predicted, the purified enzyme from a common soil fungus has a ph optimum of 5. There are numerous alternative lab activities that measure the rate of. Substrate concentration, temperature, and ph all affect the chemical reaction. Select from an extensive catalog of educational tools available for enhancing the teaching of biological subjects, including simulated testing of blood and urine, the illustration of various natural habitats, and the dissection of sterilized stool samples collected from different regions. Knowing a collection of facts about biology is beneficial only if you can use that information to understand and investigate a particular aspect of the natural world. Name the substrate and products of the peroxidasecatalyzed reaction. All organisms rely on enzymes to catalyze chemical reactions. Besides measuring the products in this lab, the amount of oxygen gas released by the reaction, enzyme activity can be measured by recording the decreasing water level in the test tube. Oct 19, 2017 ap biology syllabus, class procedures course description.

Enzyme catalysis pre lab some questions to think about before the lab. Enzymes are a form of proteins that act as catalysts to speed up chemical reactions without being consumed by the reaction. Includes access to exclusive flinnprep digital content to combine the benefits of classroom, laboratory and digital learning. Study the characteristics of an enzymemediated reaction. Enzyme catalysis overview the information will assist teachers with aspects of lab 2 that are not necessarily addressed in the lab manual. Other results for ap biology lab 21 have a blast answers. Be specific in your description, use drawings if necessary. They are able to speed up a chemical reaction without being affected, also known as a catalyst. You will use approximately 50 ml of agarose per gel depending upon your electrophoresis apparatus. In this lab, the enzyme catalase was used to break down.

Students were asked to discuss how each of the two factors they selected would affect the reaction rate. Summarize the effect of enzyme concentration on enzyme activity. Restriction enzyme student guide meets revised college board ap biology standards 4704776. Restriction enzyme cleavage of dna and electrophoresis ap. Ap biology lab materials july 2018 1 of 5 introduction lab options ap biology requires the completion of handson lab activities, and has been approved by the college board as meeting all requirements for a laboratory science course.

Ap biology syllabus, class procedures course description. They act as catalysts and affect the rate of a chemical reaction. The objective of this lab was to develop a protocol to investigate the effect of an environmental variable on the catalytic function of an enzyme. For instance, an enzyme that recognizes a four base pairs. The probability that a given enzyme will cut, or digest, a piece of dna is directly proportional to the length of its recognition site. The objective of this experiment is to develop an understanding of the role of restriction enzymes and agarose gel electrophoresis to cut and size dna. The effect of temperature and whether a seed has broken dormancy are quantified and graphed. Enzyme catalysis pre lab answers free pdf file sharing. Catalase is an enzyme that speeds up the breakdown of hydrogen peroxide into water h 2o and oxygen gas o 2. More specifically, the objective was to perform an experiment in. Two important components of enzymes are their active sites, where the actual substrates bind, and the allosteric sites, which affect the enzymes shape. The effect of ph on the rate of enzyme catalysis of catalase objectives. Students were expected to demonstrate an understanding of how environmental variables control an enzymes function. Meets ap science practices 4, 5, 6, and 7, and big idea 4.

In this lab, you will study some characteristics of enzyme action. To conduct the handson laboratory activities in this course, you will need to obtain the materials listed in. Updated urls corrections clarifications to lab procedures and equipment needs sample data tables for investigation 7 updated version of ap biology equations and formulas appendix page chapter investigation change. Peroxidase enzyme activity ap biology big idea 4, investigation an advanced inquiry lab introduction enzyme activityhow the rate of an enzymecatalyzed reaction depends on concentration, ph, temperature, metal ions, etc. The warm liver will have the best enzyme function because heat means that energy is being added and the reaction will occur faster. How to write a lab proposal, formal lab, and primers on statistics. All laboratory bench tops should be wiped down with a 10% bleach solution or disinfectant to ensure cleanliness. If a hot springs bacterial enzyme was studied and its activity data was plotted, where would it lie. You have just read in the previous section that lactaid is really the enzyme lactase. Background information enzymes serve as an aid in chemical reactions. However, teachers are not limited to only using their versions of the lab.

Four chairs and a potted plant were overturned in the center of the room, and broken pieces of glass were scattered across the floor along with several wet red drops. They are a part of the macromolecule group of protein. Enzyme catalysis ap biology flvs segment 1 2012 ap lab 2. We will be working in this lab with a representative enzyme catalase. For example, the enzyme amylase in your saliva speeds up the breakdown down of. Big genetics and information transfer 3 ap central.

Ap biology the catalase that works in liver and in red blood cells. In this lab, the enzyme catalase was used to break down hydrogen peroxide into less toxic water and oxygen gas. The new ap biology labs can be found online by clicking the link below. If another enzyme from a north sea crustacean was studied and its enzyme activity was plotted on the graph, where would it appear. The purpose of this lab is to investigate the impact of temperature, substrate concentration, enzyme concentration, and the presence of an inhibitor on the effectiveness and rate of an enzyme. Pcr is a technique where millions of copies of dna can be made from one original copy. The optimum reaction conditions are different for each enzyme. Bozeman video ap biology labs prelab questions lab 2 enzyme catalysis. In this lab, you will be given an array of materials and you will be asked to design your own experiment to test the effect of pineapple on gelatin. One function of catalase within cells is to prevent the accumulation of toxic. The main enzyme for this lab, peroxidase, is found in many different forms, with optimum phs ranging from 4 to 11 depending on the source and optimum temperatures varying from 10 to 70c.

He then explains how you can measure the rate of an enzyme. Part d instructed students to list two environmental factors that can alter the rate of enzymemediated reactions. Each test tube received 10 drops of enzyme and individually, the rate of the reaction was determined by an the appearance of o2 using an o2 sensor. Ap bio reading guides biology in focus 2nd edition. Scheduling the activities in this lab can be a bit complex. Enzymes bond with substrates and react with the substrates to release products.

How are the structure and functions of enzymes related. Abstract the purpose of this lab was to test enzyme function in various environments. Read the background information at the beginning of the lab so you understand lactose intolerance, the action of the enzyme lactase and the commercial product, lactaid. Cover the top of the agarose container flask to minimize evaporation. Ap biology lab cell respiration this investigation uses respirometry techniques to calculate the rate of oxygen consumption cellular respiration in germinating pea seeds.

The ap college board lists labs for its recommended curriculum, however, teachers are not limited to only using their versions of the lab. Scribd is the worlds largest social reading and publishing site. The enzyme had a greater reaction rate at a ph of 7. What are some good sources of the human enzyme catalase. Restriction enzyme 112 cleavage of dna and electrophoresis ap biology lab 6b see page 3 for storage instructions. The ap college board lists labs for its recommended curriculum, summarized in the publication, ap biology investigative labs. A ph of 7 seems to be the optimal level of reactivity in the peroxidase enzyme used. An enzyme is a biological catalyst that increases the rate of chemical reaction by. In the enzyme concentration lab it was determined that as the concentration of. Ap biology lab manual for teachers supplement lab 2. For example, the enzyme amylase in your saliva speeds up the breakdown down of starch the substrate into simple sugars. An inquirybased approach supplement to the first printing this document provides. The enzyme used in lab 2a and 2b catalase has four polypeptide chains, each composed of more than 500 amino acids.

Is an enzyme consumed or changed during a chemical reaction. Follow your teachers instructions regarding cleanup and disposal of all chemicals and materials used in this lab. Science is about the process of investigating, and should be a central part of your experience in ap biology. Cooperativity a form of allosteric regulation that can amplify enzyme activity. Ap biology lab investigations allow you to explore the natural world, and provide opportunities for you to choose to study what interests you most about each concept. With the peroxidase enzyme activity inquiry lab solution for ap biology, students investigate the activity of turnip peroxidase by measuring its rate of reaction with hydrogen peroxide and a natural reducing agent. Some of the recommended labs may be too expensive or too time consuming for your class. These suggestions are provided to enhance the students overall lab experience as well as their conceptual understanding. Prepare the agarose gel just before the lab and maintain it in its liquid state by placing it in a 5565c water bath.